Otolith Removal and Preparation for Microstructural Examination: A Users Manual (1991)byD.H. Secor, J.M. Dean, and E.H. Laban![]() Otolith microstructural studies exist for over 50 families and
135 species of fish and squid. A review of more than 200
investigations showed that a wide variety of techniques have been used
in otolith microstructure studies. We foresaw the need of a manual on
otolith preparation as microstructure studies become routine in life
history investigations. The purpose of our manual is to:
To accomplish these aims, detailed methods are provided on otolith removal, cleaning, storage, sectioning, polishing, and etching. We emphasize that there are many alternative methods to those described in the manual. To facilitate review of other laboratories' techniques, tables are provided which sort out published methods by author and species. ENTIRE MANUAL [Warning - This is a 34.5 Mb Document; will open in a new window] INTRODUCTION: Title Page / Foreward / Preface / Table of Contents [800 Kb; will open in a new window] CHAPTER ONE: History and Background [512 Kb; will open in a new window]
CHAPTER TWO: Position and Morphology of Otoliths in Fishes [1.9 Mb; will open in a new window]
CHAPTER THREE: Protocol Decisions [1.3 Mb; will open in a new window]
CHAPTER FOUR: Removal Techniques [3.1 Mb; will open in a new window]
CHAPTER FIVE: Cleaning, Handling, and Storage [1.0 Mb; will open in a new window]
CHAPTER SIX: Section Preparation [5.8 Mb; will open in a new window]
CHAPTER SEVEN: Etching Sections [2.0 Mb; will open in a new window]
CHAPTER EIGHT: Summary of Laboratory Protocols [1.7 Mb; will open in a new window]
CHAPTER NINE: Recommendations [416 Kb; will open in a new window] REFERENCES: Survey of Cited Literature [5.4 Mb; will open in a new window] APPENDIX I: Systematic Key [8.2 Mb; will open in a new window] APPENDICES II / III / IV: Supplies / Suppliers (1991) [2.3 Mb; will open in a new window] UPDATED SOURCES: Supplies / Suppliers (2001) [640 Kb; will open in a new window] |
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